High Frequency Ultrasound

The mechanical properties of living cells such as elasticity are highly relevant to the structural and molecular alterations induced by progression of diseases or by invasion of foreign organisms. Therefore, identification of the alternations of cells’ mechanical properties can be crucial to develop a complete knowledge of disease progression. Thus, we will have been developing multimodal imaging and analysis technologies, including a high-frequency acoustic radiation force impulse imaging and analysis method via photoacoustic detection, to probe mechanical properties of cells. In addition to the development of these technologies, we believe that it is highly important to explore biological applications of the developed imaging and analysis technologies. Thus, I will employ these technologies to measure mechanical properties of cancer cells for determination of invasiveness of cancer cells as well as for better understanding of molecular and biophysical mechanisms in the transformation of cancer cells from indolent to invasive phenotype.

Related Papers

  • Teo RD, Jae Youn Hwang, Termini J, Gross Z, Gray HB, “Fighting Cancer with CorrolesChemical Reviews2017117 (4), pp 2711–2729 [Impact Factor: 37.369]
  • Jae Youn Hwang*Jihun Kim, Jin Man Park, C. Lee, H. Jung, J.. Lee, Shung KK, “Cell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of Cell Mechanics”, Scientific Reports, Article number: 27238 , May 2016
  • S. P. Yoon, M.G Kim, C. T. Chiu, Jae Youn Hwang, H. H. Kim, Yingxiao Wang, Shung KK. “Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound”Scientific Reports Article number: 20477 , Jan 2016.
  • Jessica D. Sims, Jae Youn Hwang, Shawn Wagner, Felix Alonso-Valenteen, Chris Hanson, Jan Michael Taguiam, Richard Polo, Ira Harutyunyan, Gevorg Karapetyan, Karn Sorasaenee, Ahmed Ibrahim, Eduardo Marban, Rex Moats, Harry B. Gray, Zeev Gross, Lali K. Medina-Kauwe, “A corrole nanobiologic elicits tissue-activated MRI contrast enhancement and tumor-targeted toxicity”, Journal of Controlled Release, Nov 10;217, 2015.
  • Jae Youn Hwang, Chi Woo Yoon, Hae Gyun Lim, Jin Man Park, Sangpil Yoon, Jungwoo Lee, and K. Kirk Shung “Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells”, Ultrasonics, 63:94-101, 2015
  • Bong Jin Kang, Chang Hwan Yoon, Jin Man Park, Jae Youn Hwang*, and K. Kirk Shung “Jitter reduction technique for acoustic radiation force impulse microscopy via photoacoustic detection”, Optics Express, 20(15), 2015
  • Jae Youn Hwang, Dong Young Cheon, Hyunjune Shin, Hyun Bin Kim, and Jungwoo Lee, “Near-Field Acoustic Microbead Trapping As Remote Anchor for Single Cell Manipulation” , Applied Physics Letters, 106, 183704, 2015
  • Jae Youn Hwang, Bong Jin Kang, Changyang Lee, Hyung Ham Kim, Jinhyoung Park, Qifa Zhou, and K. Kirk Shung, “Non-contact acoustic radiation force impulse microscopy via photoacoustic detection for probing breast cancer cell mechanics”, Biomedical Optics Express, 6(1), 11-22, 2015
  • Bong Jin Kang, Jinhyoung Park, Jieun Kim, Hyung Ham Kim, Changyang Lee, Jae Youn Hwang, Ching-Ling Lien, and K. Kirk Shung, “High frequency dual mode pulsed wave Doppler imaging for monitoring the functional regeneration of adult zebrafish hearts”R. Soc. Interface, 12(103), 2015
  • Jae Youn Hwang*, Hae Gyun Lim, Chi WooYoon, Kwok Ho Lam, Sangpil Yoon, Changyang Lee, Chi Tat Chiu, BongJin Kang, Hyung Ham Kim, and K. Kirk Shung, “Non-contact high-frequency ultrasound microbeam stimulation for studying mechanotransduction in human umbilical vein endothelial cells”, Ultrasound in Medicine and biology, 40(9), 2172-82, 2014
  • Changyang Lee, Jong Seob Jeong, Jae Youn Hwang, Jungwoo Lee, and K. Kirk Shung, “Non-contact multi-particle annular patterning and Manipulation with ultrasound microbeam, Applied Physics Letters, 104, 24417, 2014
  • Jae Youn Hwang, Changyang Lee, Kwok Ho Lam, Hyung Ham Kim, Jung Woo Lee, and K. Kirk Shung, “Cell Membrane Deformation Induced by Fibronectin-coated Polystyrene Microbead in 200 MHz Acoustic Trap”, IEEE Transaction on UFFC 16(4),pp 399-406, 2014.
  • Hojong Choi, Min Gon Kim, Tommas Cummins, Jae Youn Hwang, and K. Kirk Shung, “Power MOSFET-Based Limiter For High Frequency Ultrasound Systems”, Ultrasonic Imaging, 36(4), 317-30, 2014
  • Ying Li, Jae Youn Hwang, Jung Woo Lee, and K. Kirk Shung, “Single beam acoustic tweezers: A new tool for microparticle manipulation”, Acoustic Today, 9(4), pp10-13, 2013.
  • Jae Youn Hwang, Nan Sook Lee, Changyang Lee, Kwok Ho Lam, Jonghye Woo, Ming-Yi Lin, Kassandra Kisler, Hyung Ham Kim, Qifa Zhou, Robert H. Chow, and K. Kirk Shung, “Investigating Contactless High Frequency Ultrasound Microbeam Stimulation for Determination of Invasion Potential of Breast Cancer Cells”, Biotechnology and Bioengineering, 110 (10), pp 2697-05, 2013.
  • 16.Jae Youn Hwang, Daniel L. Farkas, and Lali K. Medina-Kauwe, “Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors”, Journal of Visualized Experiments, (76) e50396, 2013.
  • Jae Youn Hwang, Sebastian Wachsmann-Hogiu, V Krishnan Ramanujan, Zeev Gross, Harry B Gray, Julia Ljubimova, Lali K. Medina-Kauwe and Daniel L. Farkas, “A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging and Chemotherapy Assessment”, Molecular Imaging and biology, 14(4), pp 431-42, 2012.
  • Jae Youn Hwang*, Jinhyung Park, Jay Lubow, David Chu, Bong Jin Kang, Daniel L. Farkas, K. Kirk Shung, and Lali K. Medina-Kauwe, “Multimodality Imaging In Vivo for Preclinical Assessment of Tumor-targeted Doxorubicin Nanoparticles”, PLOS ONE, pp e34463, 2012.
  • Jae Youn Hwang, Jungwoo Lee, Changyang Lee, Anette Jakob, Robert Lemor, Lali K. Medina-Kauwe, and K. Kirk Shung, “Fluorescence Response of Human HER2+ Cancer- and MCF-12 Normal Cells to 200 MHz Ultrasound Microbeam Stimulation: a Preliminary Study of Membranes Permeability Variation”, Ultrasonics, 52(7), pp 803-808, 2012.
  • Jae Youn Hwang, Jay Lubow, David Chu, Harry B. Gray, Zeev Gross, Lali K. Medina-Kauwe, and Daniel L. Farkas, “Investigating Photoexcitation-induced Mitochondrial Damage by Chemotherapeutic Corroles using Multimode Optical Imaging”, Journal of Biomedical Optics, 17(1), pp 015003, 2012.
  • Jae Youn Hwang, Jay Lubow, David Chu, Harry B. Gray, Zeev Gross, Daniel L. Farkas, and Lali K. Medina-Kauwe, “Photoexcitation of Tumor-targeted Corrole Induces Singlet Oxygen-mediated Augmentation of Cytotoxicity”, Journal of controlled release, 163 (3), pp 368-373, 2012.
  • Hasmik Agadjanian, David Chu, Jae Youn Hwang, Sebastian Wachsmann-Hogiu, Altan Rentsendorj, Lei Song, Vinod Valluripalli, Jay Lubow, Jun Ma, Behrooz Sharifi, Daniel L. Farkas, and Lali K. Medina-Kauwe “Chemotherapy Targeting by DNA Capture in Viral Protein Particles”, Nanomedicine, 7(3), pp 335-52, 2012.
  • Jae Youn Hwang, Jay Lubow, David Chu, Jun Ma, Hasmik Agadjanian, Harry B. Gray, Zeev Gross, Daniel L. Farkas, and Lali K. Medina-Kauwe “A Mechanistic Study of Tumor-Targeted Corrole Cytotoxicity”, Molecular Pharmaceutics, 8(6), pp 2233–2243, 2011.
  • Hasmik Agadjanian, Jun Ma, Altan Rentsendorj, Vinod Valluripalli, Jae Youn Hwang, Atif Mahammed, Anat Marom, Daniel Farkas, Harry Gray, Zeev Gross, and Lali K. Medina-Kauwe, “Tumor Detection and Elimination by a Targeted Gallium CorroleProceedings of the National Academy of Sciences, 106(15), pp 6105-6110,2009

Related Project

  • Development of a noncontact cell elastographic imaging system based on acoustic radiation force impulses for measurement of mechanics of suspended/cultured cells
  • Study on a high-frequency ultrasound technique and system for qauntification of the invaision potential of a cancer cell